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日本エネルギー学会誌 Vol. 95 (2016), No. 7

ISIJ International
belloff
オンライン版ISSN: 1882-6121
冊子版ISSN: 0916-8753
発行機関: The Japan Institute of Energy

Backnumber

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キーワードランキング

03 May. (Last 30 Days)

日本エネルギー学会誌 Vol. 95 (2016), No. 7

Sacrificial Hydrogen Production from Enzymatic Hydrolyzed Chlorella over a Pt-loaded TiO2 Photocatalyst

Masahide YASUDA, Shoichi HIRATA, Tomoko MATSUMOTO

pp. 599-604

DOI:

10.3775/jie.95.599

抄録

Chlorella is single-cell green algae which has the photosynthetic pigments chlorophylls. Through photosynthesis, it multiplies rapidly, requiring only carbon dioxide, water, sunlight, and a small amount of minerals. Since dried chlorella is composed of about 45% protein, 20% fat, 20% carbohydrate, and others, ethanol yield is low through saccharification and fermentation of chlorella. Therefore, hydrogen production from chlorella was examined through enzymatic hydrolysis and photocatalytic reaction over a Pt-loaded TiO2 photocatalyst (Pt/TiO2). Enzymatic hydrolysis of chlorella (10.0 g) was performed in phosphate buffer (60 mL) using hydrolytic enzyme (1.0 g) such as protease, cellulase, and xylanase. After hydrolysis, the precipitates were removed by centrifugation to give the supernatant aqueous enzymatic hydrolyzed solution (EH). It was found that the EH solution obtained from protease-hydrolysis contained large amounts of hydrolyzed materials as a consequence of freeze-drying. Main component in EH solution was found to be amino acids by colorimetric analysis using ninhydrin The EH solution was subjected to the sacrificial hydrogen production over a Pt/TiO2 under UV irradiation by a high-pressure Hg lamp. Hydrogen (57.9 mg) was obtained from 1.0 g of dried chlorella through protease-hydrolysis and photocatalytic hydrogen production over Pt/TiO2. This is the first report on sacrificial H2 production from chlorella.

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